Review



room temperature rt  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Cell Signaling Technology Inc room temperature rt
    Room Temperature Rt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 849 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/room+temperature+rt/DYKDDDDK+Tag+Mouse+mAb/pmc12810641-274-22-34
    Average 96 stars, based on 849 article reviews
    room temperature rt - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Saline:

    Article Title: Exosomes and soluble secretome from hormone-treated endometrial epithelial cells direct embryo implantation.
    Article Snippet: .. The membranes were blocked with 5% skim milk powder (w/v) in Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST, v/v) for 1 h at room temperature (RT) and probed with primary antibodies, mouse anti-Alix (1:1000; #2171S, Cell Signaling Technology) or mouse anti-TSG101 (1:1000; #612696, BD Biosciences) overnight at 4 C followed by horse radish peroxidase (HRP)-goat anti-mouse immunoglobulin G (1:1000, #2023- 04, Dako) for 1h at RT. .. Detection was performed treating each membrane for 2 min with enhanced chemiluminescence (BIO RAD) which provided the HRP substrate, and the signal captured by Image Lab software using ChemiDocTM XRS+ system.

    Immunolabeling:

    Article Title: A causal coding variant regulating alternative splicing of DOC2A at 16p.11.2 GWAS locus influences susceptibility to schizophrenia
    Article Snippet: .. For immunolabeling, the grids were blocked with 1% bovine serum albumin, 0.05% Triton X-100, and 0.05% Tween 20 for 5 min at room temperature (RT) and then incubated with primary antibodies (FLAG, no. 8146S, Cell Signaling Technology, 1:5; DOC2A, no. PA5-31345, Invitrogen, 1:20) at 4°C overnight. .. Following PBS washes, secondary antibodies were incubated with 10-nm colloidal gold-labeled goat anti-mouse (no. G7652, Sigma-Aldrich, 1:10) and 5-nm colloidal gold-labeled goat anti-rabbit (no. G7277, Sigma-Aldrich, 1:20) for 2 hours at RT.

    Incubation:

    Article Title: A causal coding variant regulating alternative splicing of DOC2A at 16p.11.2 GWAS locus influences susceptibility to schizophrenia
    Article Snippet: .. For immunolabeling, the grids were blocked with 1% bovine serum albumin, 0.05% Triton X-100, and 0.05% Tween 20 for 5 min at room temperature (RT) and then incubated with primary antibodies (FLAG, no. 8146S, Cell Signaling Technology, 1:5; DOC2A, no. PA5-31345, Invitrogen, 1:20) at 4°C overnight. .. Following PBS washes, secondary antibodies were incubated with 10-nm colloidal gold-labeled goat anti-mouse (no. G7652, Sigma-Aldrich, 1:10) and 5-nm colloidal gold-labeled goat anti-rabbit (no. G7277, Sigma-Aldrich, 1:20) for 2 hours at RT.

    Article Title: In vitro maturation using porcine follicular fluid-derived exosomes as an alternative to the conventional method.
    Article Snippet: The lysate was loaded onto a 4–12 % Bis-Tris gel for 20 min at 200 V. The separated proteins were transferred onto a PVDF membrane at 20 V for 1 h using the Mini Blot Module transfer system (Thermo). .. The membranes were blocked with TBST, containing 5 % skim milk, for 30 min at room temperature (RT) and then incubated with the antiFlotillin1 (18634, Cell Signaling Technology), anti-CD63 (LSC204227-600, Isbio), and anti-tumor susceptibility gene 101 (TSG101) primary antibodies (MA1-23296, Thermo Fisher Scientific; diluted 1:1000 in blocking buffer) for 1 h at RT. .. The membranes were washed thrice, for 10 min each time, with TBST and then incubated with the anti-mouse IgG, anti-rabbit IgG, and anti-goat IgG secondary antibodies (Sigma-Aldrich; diluted 1:5000 in the blocking solution) for 1 h at RT.

    Article Title: Myoglobin-derived iron causes wound enlargement and impaired regeneration in pressure injuries of muscle
    Article Snippet: For blotting, 30 μg samples were separated by SDS- PAGE (Bio- Rad Laboratories Inc, CA) and transferred to PVDF membranes (Bio- Rad Laboratories Inc). .. The PVDF membranes were blocked with 5% blotting grade blocker (Bio- Rad Laboratories Inc) at room temperature (RT) for 1 hr and then incubated with the primary antibodies (anti- myoglobin antibody, 25919S, Cell Signaling Technology) overnight at 4°C. ..

    Blocking Assay:

    Article Title: In vitro maturation using porcine follicular fluid-derived exosomes as an alternative to the conventional method.
    Article Snippet: The lysate was loaded onto a 4–12 % Bis-Tris gel for 20 min at 200 V. The separated proteins were transferred onto a PVDF membrane at 20 V for 1 h using the Mini Blot Module transfer system (Thermo). .. The membranes were blocked with TBST, containing 5 % skim milk, for 30 min at room temperature (RT) and then incubated with the antiFlotillin1 (18634, Cell Signaling Technology), anti-CD63 (LSC204227-600, Isbio), and anti-tumor susceptibility gene 101 (TSG101) primary antibodies (MA1-23296, Thermo Fisher Scientific; diluted 1:1000 in blocking buffer) for 1 h at RT. .. The membranes were washed thrice, for 10 min each time, with TBST and then incubated with the anti-mouse IgG, anti-rabbit IgG, and anti-goat IgG secondary antibodies (Sigma-Aldrich; diluted 1:5000 in the blocking solution) for 1 h at RT.



    Similar Products

    99
    Thermo Fisher room temperature rt
    Room Temperature Rt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/room+temperature+rt/Triton+X-100/10__1016_slash_j__mtbio__2026__103375-75-20-14
    Average 99 stars, based on 1 article reviews
    room temperature rt - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    95
    Vector Laboratories room temperature rt
    Room Temperature Rt, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/room+temperature+rt/R%2ET%2EU%2E+Animal-Free+Blocker+and+Diluent+(AFB)/bio_rxiv__64898__2026__04__17__719285-35-15-22
    Average 95 stars, based on 1 article reviews
    room temperature rt - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    86
    Affinity Biosciences room temperature rt
    Room Temperature Rt, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/room+temperature+rt/anti+room+temperature/pmc13019769-53-15-43
    Average 86 stars, based on 1 article reviews
    room temperature rt - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    98
    Bio-Rad room temperature rt
    Room Temperature Rt, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/room+temperature+rt/Tween+20/pm41870795-68-64-32
    Average 98 stars, based on 1 article reviews
    room temperature rt - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    98
    Proteintech room temperature rt
    Room Temperature Rt, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/room+temperature+rt/GAPDH+Monoclonal+antibody/pm41782112-112-11-24
    Average 98 stars, based on 1 article reviews
    room temperature rt - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    95
    Santa Cruz Biotechnology room temperature rt
    Room Temperature Rt, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/room+temperature+rt/ADAR1+Antibody/bio_rxiv__64898__2026__01__29__701210-280-19-29
    Average 95 stars, based on 1 article reviews
    room temperature rt - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc room temperature rt
    Room Temperature Rt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/room+temperature+rt/DYKDDDDK+Tag+Mouse+mAb/pmc12810641-274-22-34
    Average 96 stars, based on 1 article reviews
    room temperature rt - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results